Award details

The role of osteoblast AMPA receptors in stimulation of bone formation

Reference	S18293/2
Principal Investigator / Supervisor	Professor T M Skerry
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Medicine and Biomedical Science
Funding type	Research
Value (£)	137,693
Status	Completed
Type	Research Grant
Start date	16/01/2006
End date	15/10/2007
Duration	21 months

Abstract

The most intractable problem in skeletal pathology is stimulation of bone formation after loss. In pilot studies, we discovered that surprisingly, low but not high doses of antagonists of AMPA-type glutamate receptors stimulate expression of osteoblast phenotypic markers and bone formation in vitro and in vivo. We will test the hypothesis that low doses of antagonists that bind to the thiazide- sensitive anti-desensitisation site of AMPA receptors exert agonist effects, initiating osteogenic responses. We will determine subgroups of AMPA receptors expressed in bone, marrow and osteoblasts, and the characteristics of ligand and putative antagonist binding to osteoblasts. We will also measure effects of antagonists at different doses on intracellular calcium concentration, c-fos for expression and AP-1 activity, and in vivo, on bone mass in rats after ovariectomy and in response to mechanical loading.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

S18293 The role of osteoblast AMPA receptors in stimulation of bone formation

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