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Oocyte-somatic cell interactions: identification of novel oocyte secreted proteins

ReferenceS17232
Principal Investigator / Supervisor Professor Morag Hunter
Co-Investigators /
Co-Supervisors
Professor Robert Webb
Institution University of Nottingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 305,596
StatusCompleted
TypeResearch Grant
Start date 01/07/2002
End date 28/02/2006
Duration44 months

Abstract

It is becoming clear that follicular development is dependent upon and:or influenced by the oocyte. However, the mechanisms through which the oocyte exerts its effects are ill defined. We will investigate the influence of known oocyte secreted factors on somatic cell function in vitro and define the temporal expression of other likely candidates in somatic-germ cell interactions (e.g. GDF-9, BMP-15). The ovarian autotransplant gilt model will be exploited to obtain numbers of synchronised early follicles. We will then use proteomics to identify specific proteins secreted by, or expressed in oocytes at defined stages of development. Novel oocyte proteins secreted in a temporal manner that are likely to be key regulators of follicle growth will be identified, sequenced and biological activity assessed.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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