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Functional analysis of a preproneuropeptide gene (Dtk) from the Drosophila genome

Reference	S15562
Principal Investigator / Supervisor	Professor Elwyn Isaac
Co-Investigators / Co-Supervisors	Professor Alison Ashcroft, Dr Alan Shirras, Dr Richard Siviter
Institution	University of Leeds
Department	Inst of Integrative & Comparative Biolog
Funding type	Research
Value (£)	205,588
Status	Completed
Type	Research Grant
Start date	02/01/2002
End date	02/01/2005
Duration	36 months

Abstract

The sequenced genome of D. melanogaster has revealed the prepropeptide genes for many neuropeptides believed to be involved in neurotransmission and endocrine signalling. The role in development, behaviour and gut physiology of one of these genes, coding for a family of tachykinin peptides (Dtk), will be studied using P-element-induced mutants, conditional gene silencing and ectopic expression. Interspecific comparison of the Dtk gene will identify functionally important sequences that have been evolutionarily conserved. A novel immunocapture-mass spectrometric assay will be developed for processed DTK peptides to monitor pro-hormone processing during development and in different tissues. The project may be extended to the proctolin preprohormone gene (Pro), if time permits.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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