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Expression analyses in the obligate intracellular bacterium Lawsonia intracellularis for characterisation of in vivo-induced genes

ReferenceS14219
Principal Investigator / Supervisor Professor David Smith
Co-Investigators /
Co-Supervisors
Dr Jackie McCluskey
Institution University of Edinburgh
DepartmentMedical Microbiology
Funding typeResearch
Value (£) 241,904
StatusCompleted
TypeResearch Grant
Start date 01/06/2001
End date 30/09/2003
Duration28 months

Abstract

Gene expression in Lawsonia intracellularis will be examined during infection in vitro in intestinal cell lines and in vivo by use of a promoter probe plasmid system. An adaptation of IVET/DFI methods with CAT and GFP as dual reporters in a broad host range plasmid will be applied to this highly fastidious obligate intracellular pathogen. Chloramphenicol and/or ampicillin will be used to select and assess gene expression. The system will be employed to monitor expression of an identified Lawsonia gene (a haemolysin which we have characterised) as well as global examination of expression. These investigations will characterise loci which are essential and/or up-regulated during infection. The defining of these determinants has multiple downstream applications.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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