Award details

Expression analyses in the obligate intracellular bacterium Lawsonia intracellularis for characterisation of in vivo-induced genes

Reference	S14219
Principal Investigator / Supervisor	Professor David Smith
Co-Investigators / Co-Supervisors	Dr Jackie McCluskey
Institution	University of Edinburgh
Department	Medical Microbiology
Funding type	Research
Value (£)	241,904
Status	Completed
Type	Research Grant
Start date	01/06/2001
End date	30/09/2003
Duration	28 months

Abstract

Gene expression in Lawsonia intracellularis will be examined during infection in vitro in intestinal cell lines and in vivo by use of a promoter probe plasmid system. An adaptation of IVET/DFI methods with CAT and GFP as dual reporters in a broad host range plasmid will be applied to this highly fastidious obligate intracellular pathogen. Chloramphenicol and/or ampicillin will be used to select and assess gene expression. The system will be employed to monitor expression of an identified Lawsonia gene (a haemolysin which we have characterised) as well as global examination of expression. These investigations will characterise loci which are essential and/or up-regulated during infection. The defining of these determinants has multiple downstream applications.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

S14219/2 Expression analyses in the obligate intracellular bacterium Lawsonia intracellularis for characterisation of in vivo-induced genes

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