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Identification of regulators of in vivo expressed genes of Actinobacillus pleuropneumoniae

Reference	S13705
Principal Investigator / Supervisor	Professor Paul Langford
Co-Investigators / Co-Supervisors	Professor John Kroll, Professor Andrew Rycroft, Dr Brian Sheehan
Institution	Imperial College London
Department	Reproductive and Developmental Biology
Funding type	Research
Value (£)	253,184
Status	Completed
Type	Research Grant
Start date	01/03/2001
End date	01/03/2004
Duration	36 months

Abstract

We propose to construct promoter probe plasmids using GFP and Xy1E as reporters of gene expression for use in identifying regulators of in vivo expressed genes of Actinobacillus pleuropneumoniae (APL). Transposon mutagenesis of APL transformed with the reporter plasmids containing promoters of in vivo expressed (in vitro repressed) genes will allow the selection of mutants with interruptions in putative regulatory genes (repressors). The sequence and prevalence of such genes amongst APL serotypes and related organisms will be determined. Comparison of the virulence potential of mutants interrupted in a gene of interest with the wild type in experimental infection will determine whether such genes have a role in virulence.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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