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Use of the yeast signal sequence trap to characterise scarce membrane and secreted antigens of helminth parasites
Reference
S12982
Principal Investigator / Supervisor
Professor Alan Wilson
Co-Investigators /
Co-Supervisors
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
160,356
Status
Completed
Type
Research Grant
Start date
01/02/2000
End date
01/05/2003
Duration
39 months
Abstract
Antigens exposed on membranes, or secreted (M/S) are the likely mediators of protective immunity against helminth parasites. We propose to use the yeast signal sequence trap to isolate cDNAs encoding M/S proteins from schistosomes. Random primed cDNAs from three different larval stages will be introduced into a mutant yeast strain. Those cDNAs restoring the ability of yeast clones to grow on sucrose will be sequenced. The expressed recombinant proteins from selected clones will be purified to raise antisera for immunolocalisation studies, in order to confirm their M/S origin. The antigens we find may be vaccine candidates, and the methods we establish will be applicable to other helminths.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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