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The role of peroxisomal proliferator-activated receptors in regulating lipid metabolism in fish

Reference	S12045
Principal Investigator / Supervisor	Professor Douglas Tocher
Co-Investigators / Co-Supervisors	Dr Michael Leaver
Institution	University of Stirling
Department	Institute of Aquaculture
Funding type	Research
Value (£)	183,016
Status	Completed
Type	Research Grant
Start date	16/12/1999
End date	16/12/2002
Duration	36 months

Abstract

The roles of PPARs in regulating lipid metabolism in marine fish will be studied. Plaice PPAR genes will be cloned and characterised, and tissue distribution of PPARs will be determined by Northern analysis. Activation profiles to peroxisome proliferators (PP), fatty acids and their derivatives, and sequence requirements for PPAR-dependent regulation will be determined in cell transfection and DNA footprinting experiments, using a plaice PPAR- responsive gene promoter and plaice PPAR cDNAs. The in vivo response of marine fish to PP, fatty acids and their derivatives will be investigated by determining fatty acid peroxisomal alpha-oxidation, microsomal omega-hydroxylation and desaturation. Plaice fatty acid desaturase sequences will be isolated using RT-PCR.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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