Award details

Molecular cloning and functional characterisation of rainbow trout interferons

Reference	S11575
Principal Investigator / Supervisor	Professor Kin-Chow Chang
Co-Investigators / Co-Supervisors	Professor Chris Secombes
Institution	University of Glasgow
Department	Veterinary School
Funding type	Research
Value (£)	92,558
Status	Completed
Type	Research Grant
Start date	01/07/1999
End date	01/07/2001
Duration	24 months

Abstract

Cytokines are simple but critical secreted polypeptides that act as signalling molecules between cells of the immune system. To date no major fish cytokines, including interferons (IFNs), have been cloned, partly due to a lack of nucleotide sequence homology between fish and mammals. To overcome this problem, we propose to clone rainbow trout IFNs by subtractive hybridisation between lambda ZAP-Express (Stratagene) cDNA libraries, derived from uninduced and poly(IC) double stranded RNA induced rainbow trout RTG-2 cells, which secrete IFNs at high levels upon stimulation. Putative IFN clones will be subjected to detailed molecular and in vitro functional characterisation, including IFN protection bioassays.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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