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Award details
Development of alphavirus vectors for use in fish
Reference
S11508
Principal Investigator / Supervisor
Dr Daniel Todd
Co-Investigators /
Co-Supervisors
Dr Jonathan Weston
Institution
Queen's University of Belfast
Department
Agriculture & Environmental Science
Funding type
Research
Value (£)
158,005
Status
Completed
Type
Research Grant
Start date
01/08/1999
End date
23/03/2003
Duration
44 months
Abstract
The RNA replication and translation elements of the salmon pancreas disease virus genome will be used to construct two alphavirus vectors that will find application in the development of rDNA-based fish virus vaccines. The first vector will function by transfecting cultured fish cells with RNA, in vitro transcribed from the plasmid vector. The second, a layered DNA/RNA vector, will function by transfecting cultured fish cells or fish tissue in vivo with a DNA plasmid that incorporates the self-amplifying virus RNA replicon. The abilities of the vectors to express the luciferase gene in cultured fish cells at temperatures below 20 degrees C and in fish tissue following intramuscular inoculation of salmonid fish will be evaluated.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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