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Ultrastructural analysis of confocally visualised and physiologically characterised synapses in hippocampus
Reference
S11211
Principal Investigator / Supervisor
Professor Mike Stewart
Co-Investigators /
Co-Supervisors
Institution
Open University
Department
Life, Health & Chemical Sciences
Funding type
Research
Value (£)
200,008
Status
Completed
Type
Research Grant
Start date
01/10/1999
End date
01/10/2002
Duration
36 months
Abstract
The study of synaptic plasticity in the mammalian brain has been hindered by the constraints that electrophysiological techniques sample an indeterminate number of synapses, while morphological techniques sample synapses which may or may not have been activated. Recent developments in confocal microscopy using intracellular Ca2+ indicators now permit physiological activity to be monitored at individual visualised dendritic spines in the CNS. In this study Ca2+ indicators will be co-injected with biocytin to enable subsequent electron microscopic investigation of the synaptic input on individual physiologically characterised hippocampal spines, permitting for the first time an unambiguous correlation of synaptic morphology and function.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
BioImaging (BI) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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