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Visualisation of retrovirus particles: study of virus production and infection processes

Reference	S11200
Principal Investigator / Supervisor	Professor Mary Collins
Co-Investigators / Co-Supervisors	Dr Yasuhiro Takeuchi
Institution	University College London
Department	Immunology and Molecular Pathology
Funding type	Research
Value (£)	191,431
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/12/2001
Duration	34 months

Abstract

The aim of this project is to gain a better understanding of the mechanism by which retroviral particles bud from producer cells and infect target cells by visualisation of retrovirus particles using fluorescence microscopy. We will establish methods to immunostain virus particles in fixed samples using antibodies against virus core and envelope proteins and to detect single RNA genomes by fluorescent in situ hybridisation (FISH). Retrovirus proteins will be tagged with Aequorea victoria green fluorescent proteins (GFPs), which will allow us to observe virus production and infection processes in real time. Virus assembly and disassembly in producer and target cells will be observed by colocalisation between differentially coloured virus components. Virus-cell interaction and virus translocation will be examined by co- visualisation of virus and cell components.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	BioImaging (BI) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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