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Serotype specificity of Salmonella-host interactions in vivo

Reference	S10274
Principal Investigator / Supervisor	Dr Timothy Wallis
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	Div of Microbiology Compton
Funding type	Research
Value (£)	157,328
Status	Completed
Type	Research Grant
Start date	01/10/1998
End date	28/05/2003
Duration	44 months

Abstract

The aim of this study is to further characterise the biological basis of Salmonella serotype-host specificity in cattle and pigs. Bovine and porcine ligated ileal loops will be infected with wild-type and defined mutant strains of different Salmonella serotypes and the host cells with which the Salmonellas interact with will be identified using immunohistocytochemistry in conjunction with fluorescent and confocal laser microscopy. The method of bacterial translocation from the intestinal mucosa to the systemic organs will be investigated by cannulation of blood and lymph vessels and by photon-counting imaging of bioluminescent bacteria following infection of ligated ileal loops. The effect of infection on the host will be determined with respect to Salmonella-induced cell death, cytokine production and influx of inflammatory cells.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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