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Identification of in vivo expressed genes of Actinobacillus pleuropneumoniae and their role in virulence

Reference	S09628
Principal Investigator / Supervisor	Professor Paul Langford
Co-Investigators / Co-Supervisors	Professor John Kroll, Professor Andrew Rycroft
Institution	Imperial College London
Department	Imperial College London Department
Funding type	Research
Value (£)	161,805
Status	Completed
Type	Research Grant
Start date	16/02/1999
End date	15/11/2002
Duration	45 months

Abstract

We plan to make fusions of fragmented chromosomal DNA from Actinobacillus pleuropneumoniae (APL) serotype 1 strain 4074 with the gene encoding protein III of filamentous phage. Immunogenic proteins specific to infection will then be identified from phage clones reacting with convalescent serum which has been previously absorbed with control phage in addition to whole cells and culture supernatants (as source of ApxI and ApxII) derived from in vitro grown APL. The prevalence of the immune response to those in vivo specific epitopes and the distribution of their encoding genes within APL serotypes will be determined. Genes encoding the new immunogenic proteins of interest will be cloned, inactivated by insertion of a kanamycin cassette and used to construct isogenic defined APL mutants. Comparison of the virulence potential of these mutants with their parent strains in experimentally infected pigs will determine whether such proteins have a role in virulence.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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