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Interactions between the regulatory proteins of the thin filament in striated muscle
Reference
S09231
Principal Investigator / Supervisor
Professor John Sparrow
Co-Investigators /
Co-Supervisors
Dr Justin Molloy
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
200,230
Status
Completed
Type
Research Grant
Start date
15/10/1998
End date
15/10/2001
Duration
36 months
Abstract
The aim is to determine the nature of detailed molecular interactions between the proteins involved in thin filament regulation of striated muscles, using the only genetic system in which both direct selection for mutants affecting this system and their biochemical/physiological analysis are currently possible - namely the indirect flight muscles (IFMs) of Drosophila melanogaster. Mutations in the troponin and tropomyosin genes which disrupt thin filament regulation or suppress the effects of such mutations will be sought. Sites of interaction between the proteins in this complex will be identified by sequencing the mutant genes. The roles of the different proteins or protein domains in calcium or stretch activation will be dissected by biochemical and physiological analyses of the mutants including in vitro motility and skinned fibre mechanics.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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