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Genetic vaccination targeted to cutaneous dendritic cells

Reference	S08160
Principal Investigator / Supervisor	Professor Gordon Harkiss
Co-Investigators / Co-Supervisors	Professor John Hopkins
Institution	University of Edinburgh
Department	Veterinary Pathology
Funding type	Research
Value (£)	193,353
Status	Completed
Type	Research Grant
Start date	05/01/1998
End date	05/01/2001
Duration	36 months

Abstract

The aim of the work is to target a model of virus DNA construct (p42k) to ovine cutaneous dendritic cells using a gene gun to administer the vaccine. The first aim will be to determine the efficiency of DNA uptake and expression by Langerhans cells (LC) in the skin and dendritic cells (DC) in the draining lymph nodes. The degree of afferent lymph DC antigen uptake and functional antigen carriage will be assessed after both primary and secondary vaccination. The effects of co-administering vectors encoding IFN gamma IL-10 and GM- CSF on the ability of transfected DC to activate T cells - and to modulate humoral or cell- mediated responses to p42k - will be investigated. The results of the work will aid the optimisation of DNA vaccination strategies and hasten the introduction of this technology into species of veterinary importance.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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