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Cloning expression and manipulation of key enzymes catalysing ecdysteroid transformations in insects

Reference	S07959
Principal Investigator / Supervisor	Professor Huw Rees
Co-Investigators / Co-Supervisors	Dr David O'Reilly, Dr Philip Turner
Institution	University of Liverpool
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	158,803
Status	Completed
Type	Research Grant
Start date	01/03/1998
End date	01/03/2001
Duration	36 months

Abstract

A major route of inactivation of moulting hormones (ecdysteroids) in Lepidopteran midgut is by ecdysone oxidase-catalysed conversion into the 3-dehydro derivatives, which are then reduced by 3-dehydroecdysone 3alpha-reductase, irreversibly yielding inactive 3-epi-ecdysterioids (3alpha-hydroxy). The overall aim of the project is to investigate the developmental control of these enzymes and to elucidate their physiological significance. The specific aims are: (i) to clone cDNAs for these enzymes from Spodoptera littoralis, (ii) to investigate developmental control of enzyme expression, (iii) to generate recombinant baculoviruses expressing the respective cDNAs, (iv) to evaluate the effects on host endocrinology of enzyme overproduction in vivo, (v) to undertake preliminary evaluation of the insecticidal properties of the recombinant viruses, and (vi) to produce recombinant enzyme proteins for further study (long term).

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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