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PhACE: phenotypic analysis of C. elegans strains using confocal imaging of neuronal and muscular function

Reference	S07697
Principal Investigator / Supervisor	Professor Lindy Holden-Dye
Co-Investigators / Co-Supervisors	Professor John Chad, Professor Robert J. Walker
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	47,256
Status	Completed
Type	Research Grant
Start date	01/05/1997
End date	01/05/1998
Duration	12 months

Abstract

The genome of C. elegans will be sequenced within the next two years and, as the nervous system is uniquely well-defined, it is providing a superb model to address the genetic bases for neural signalling. However, the strength of the molecular and genetic approach is not matched by physiological analyses, leading to poor resolution of phenotypic differences. We propose to help redress this imbalance by applying ratiometric confocal microscopy to calcium imaging of identified neurones and muscle in C. elegans. Preliminary experiments have demonstrated the feasibility of this approach and the project will seek to optimise conditions, map responses to excitatory neurotransmitters, and to identify abnormal responses in unresolved mutants. The provision of this refined phenotypic analysis will provide a powerful new tool to delineate the role of genes in neural signalling.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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