Award details

Characterisation and quantification of gene expression in the mammalian circadian clock

Reference	S07073
Principal Investigator / Supervisor	Dr P Powell
Co-Investigators / Co-Supervisors	Dr Iain Campbell, Professor Clive Coen
Institution	King's College London
Department	Institute of Psychiatry
Funding type	Research
Value (£)	349,703
Status	Completed
Type	Research Grant
Start date	01/01/1997
End date	31/03/2003
Duration	75 months

Abstract

The circadian pacemaker in mammals in located in the suprachiasmatic nuclei (SCN) but the nature of this pacemaker remains obscure despite the discovery of clock genes in invertebrates. This project is designed to identify compatible genes in the mammalian SCN. Using the technique of differential display on tissue obtained at specific points in the free- running cycle, we will clone and sequence genes which are expressed in the circadian manner within the SCN. The neuroanatomical distribution and circadian pattern of expression of the candidate genes will be examined by in situ hybridisation. In addition, the effects of phase- shifting stimuli on the kinetics of gene expression in the SCN will be established by serial analysis of gene expression (SAGE). This research is timely and will provide a fundamental advance in elucidating circadian clock mechanisms.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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