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Analysis of the replication of the avian coronavirus infectious bronchitis virus (IBV)

ReferenceS03905
Principal Investigator / Supervisor Dr Thomas David Kay Brown
Co-Investigators /
Co-Supervisors
Professor Ian Brierley
Institution University of Cambridge
DepartmentPathology
Funding typeResearch
Value (£) 15,178
StatusCompleted
TypeResearch Grant
Start date 01/05/1995
End date 01/05/1998
Duration36 months

Abstract

A programme for the study of avian coronavirus IBV replication is described. RNA- dependent polymerase (1a/b) polyprotein) function will be studied with emphasis on polyprotein processing and on functional studies using expression of 1a/b to replicate and transcribe defective RNAs containing appropriate cis-acting signals. Genetic manipulation of IBV using recombination of synthetic defective RNAs will be developed and the ability of T7 transcription systems to produce 27.6kb RNA required for infectious cDNA clone systems will be tested. The role of the long 5' UTR of IBV genomic RNA in its translational initiation will be studied.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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