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Structure-function relationships in autocrine inhibitors of bovine milk secretion

Reference	S02469
Principal Investigator / Supervisor	Professor H Morris
Co-Investigators / Co-Supervisors	Professor Anne Dell
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	82,064
Status	Completed
Type	Research Grant
Start date	01/02/1995
End date	01/02/1998
Duration	36 months

Abstract

The project will analyse the structure of two bovine milk proteins which act as autocrine inhibitors of milk secretion. Amino acid and carbohydrate sequences will be analysed by electrospray and fast atom bombardment mass spectrometry, together with linkage analysis and gas phase sequencing. Peptides and glycopeptides generated during structural analysis will be tested for their ability to inhibit milk constituent synthesis in cultures of differentiated mammary epithelial cells. Bioactive peptides will also be tested in lactating cows. Antibodies against protein fragments which are specific for one or other inhibitor will be used to measure their milk concentration during lactation, and to screen milk proteins for inactive inhibitor precursors or products.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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