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Newcastle disease virus gene expression in baculovirus: formation of virus-like particles and replication of RNA

Reference	S02319
Principal Investigator / Supervisor	Professor P Emmerson
Co-Investigators / Co-Supervisors	Dr Anthony Samson
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	217,019
Status	Completed
Type	Research Grant
Start date	01/01/1995
End date	25/05/1998
Duration	41 months

Abstract

Complementary DNA to Newcastle disease virus genes cloned into baculovirus vectors will be expressed in insect cells. The insect cells will also be co-infected with a recombinant baculovirus expressing a cloned DNA fragment which on transcription and cleavage at ribozyme sites will yield a truncated version of the viral RNA genome. The production in the cells of NDV virus-like particles (VLPs) will be monitored and the yield optimised. VLPs will be purified and their efficacy as poultry vaccines assessed. The ability of the expressed NDV proteins to replicate the RNA will also be studied.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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