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Structure, function and regulation of granule chymases in mucosal mast cells from normal and parasitised sheep
Reference
S01356
Principal Investigator / Supervisor
Professor Hugh Miller
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Veterinary Clinical Studies
Funding type
Research
Value (£)
336,030
Status
Completed
Type
Research Grant
Start date
01/11/1993
End date
01/11/1997
Duration
48 months
Abstract
The massive recruitment of mucosal mast cells (MMC) during intestinal nematodiasis is considered to be predominantly IL-3- dependant, but the expression of abundant granule-specific chymases may be modulated by other cytokines. The ovine MMC-derived chymase, SMCP (Mr 27,000), is expressed by cultured bone marrow mast cells together with Chy 31, a novel (Mr 31,000) putative chymase. We will compare the primary structures of SMCP and Chy 31 by molecular cloning and, using DNA probes and monoclonal antibodies, investigate the influence of IL-3 and of nematodiasis on chymase expression by different mast cell subpopulations. Characterisation of two novel plasma inhibitors which block the binding of polyclonal antibodies to SMCP may lead to improved detection of this chymase in body fluids.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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