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Cyclic AMP-dependent protein kinase structure, function and regulation in mammary gland during pregnancy and lactation

ReferenceS01106
Principal Investigator / Supervisor Dr Mike Munday
Co-Investigators /
Co-Supervisors
Institution University College London
DepartmentDept of Pharmaceutical and Biological Ch
Funding typeResearch
Value (£) 188,426
StatusCompleted
TypeResearch Grant
Start date 04/10/1993
End date 31/05/1997
Duration43 months

Abstract

Tissue-specific hormonal responses are associated with atypical properties of cAMP-dependent protein kinase (cAMP-PK) in mammary gland. We aim to characterise the molecular basis for the uniqueness of mammary cAMP-PK by : (1) Determination of the primary structure of its catalytic subunit and comparison of the C subunit structure and properties with those of known isozymes. (2) Identification and structural analysis, via copurification and related techniques, of C subunit-specific binding proteins and activity modulating factors unique to this tissue. (3) Measurement, using cDNA probes and antibodies, of C subunit and modulator mRNA and protein expression throughout pregnancy and lactation and in response to hormonal manipulation.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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