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Retinol and odour binding proteins (RBP,OBP) and their membrane bound receptors
Reference
S00359
Principal Investigator / Supervisor
Professor John B C Findlay
Co-Investigators /
Co-Supervisors
Professor Asipu Sivaprasadarao
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
172,220
Status
Completed
Type
Research Grant
Start date
01/04/1993
End date
15/08/1997
Duration
53 months
Abstract
Our original discovery that many cells contain plasma membrane receptors for serum RBP has now been extended by the demonstration of specific membrane-bound receptors for urinary and nasal mucosa OBP. We aim to (i) clone the receptors for RBP using an expression cloning approach and use hybridisation methods to clone the presumably related OBP receptor, (ii) investigate using protein engineering and the construction of chimaeric proteins, the sites on RBP and OBP responsible for specific interaction with their respective receptors, (iii) study the cellular uptake process for retinol using expressed cellular RBP and (iv), use NMR to study the role and conformational changes of the active site Trp in the apo-holo RBP transition.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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