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Characterisation of higher plant photolyase genes and analysis of their role in protection from ultraviolet-B radiation
Reference
RSP07843
Principal Investigator / Supervisor
Dr Clifford Bray
Co-Investigators /
Co-Supervisors
Dr Soheila Mackerness
,
Professor Brian Thomas
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
195,328
Status
Completed
Type
Research Grant
Start date
01/04/1997
End date
01/04/2000
Duration
36 months
Abstract
Photoreactivation, the most effective DNA repair system in higher plants, involves the action of at least two classes of photolyase repair enzymes. We have isolated via a PCR approach, a putative cyclobutane pyrimidine dimer (CPD) photolyase which has been characterised by nucleotide sequence analysis (Accession No X99301). We propose to employ a combined molecular and genetic approach to (i) identify and fully characterise Arabidopsis CPD and (6- 4) photoproduct photolyase genes at the molecular level, (ii) investigate the effects of varying UV-B, light and temperature on photolyase gene expression and morphological and physiological features of transgenic plants and (iii) determine the function and role of photolyases in protection of plants against damage by UV-B irradiation.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Resource Allocation and Stress in Plants (RSP) [1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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