Award details

Real-time PCR and high throughput sequencing facilities for biomedical research microbiology and cell biology

Reference	REI20575
Principal Investigator / Supervisor	Professor Nicholas Mann
Co-Investigators / Co-Supervisors	Professor John Davey, Professor Andrew Easton, Professor Michael Lord, Professor John Murrell, Dr Paul Norris, Professor Lynne Roberts, Professor Elizabeth Wellington
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	78,274
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/06/2004
Duration	9 months

Abstract

Funds are sought from BBSRC to make a 49 per cent contribution towards Real Time PCR and high-throughput sequencing equipment for a very wide range of studies in biomedical research, molecular microbiology and molecular cell biology. The balance of funding will be provided by a local charity and by the equipment manufacturer. The applicants are all highly-regarded researchers with well-established programmes and a good track record of BBSRC funding. Specifically we seek an ABI Prism 7000 Sequence Detection system for Real Time PCR quantification of gene expression and an ABI Prism 3100 Genetic Analyser for automated DNA sequencing. We currently have no capability for quantitative PCR and our sequencing is all run on a single ABI 3100 which is already operating to capacity. Addition of a second sequencer will allow the current instrument to be used exclusively for conventional sequencing on POP6 polymer with an average read-length of 650 bases, while the new instrument is set up with alternative capilliaries and POP4 polymer to allow long sequencing reads of over 1 kb or very rapid sequencing of short sequences of up to 500 bases. The equipment will be located within an existing departmental molecular biology service and will enhance and extend the capabilities of the service. As such, it will support the research programmes not only of the applicants but of a very wide range of researchers in the Department. It will offer postgraduate students and post-doctoral researchers the opportunity to train and gain experience with state-of-the-art equipment for genomic and post-genomic research. The research programmes to be supported by the facility include work on bacterial and viral infection (S. aureus, E. coli, L. monocytogenes, S. typhimurium, pneumoviruses, rotaviruses and adenoviruses); on microbial molecular ecology including photosynthetic marine cyanobacteria, mineral biotechnology and methane and other C1 metabolism; on the secretory and endocytotic pathways of eukaryotes and their exploitation by ribotoxic proteins such as ricin; and on cell signalling via G-protein coupled receptors and their significance in disease states such as diabetes, obesity, abnormal labour, cardiovascular diseases and cancer.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2003 (RE2) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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