Award details

Quantitative proteomics using isotope tagging and additional LC MS/MS capacity for the Cambridge Centre for Proteomics

Reference	REI20564
Principal Investigator / Supervisor	Professor Kathryn Lilley
Co-Investigators / Co-Supervisors	Dr David Mark Carrington, Professor Paul Dupree, Professor David Ellar, Dr Antony Jackson, Dr Paul Kemp, Professor Ernest Laue, Professor Peter Leadlay, Professor George Salmond, Professor Christopher Smith, Dr Nancy Standart, Dr Martin Welch
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	150,000
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2004
Duration	12 months

Abstract

In this proposal we request funding to purchase an additional mass spectrometer capable of operating as LC-MS/MS from Applied Biosystems. The rationale behind this request is threefold: To provide CCP with sufficient LC-MS/MS capacity to meet the demands of local researchers who largely form the set of co-applicants of this proposal. Detailed descriptions of the research to be facilitated by the purchase of this equipment are listed in the case for support. To ensure CCP remains at the forefront of recent technological advances in the field of quantitative proteomics in situations where samples are unsuitable for 2D PAGE approaches. This will benefit existing collaborators from the Arabidopsis and Drosophila communities who use CCPs facilities as part of BBSRC IGF Initiative. To enable CCP to expand their existing collaborations with Applied Biosystems by evaluating and developing the use of QSTAR system in conjunction with integral membrane protein samples, developing further cICAT and its integration into a routine core facility setting and optimisation of the system using a variety of staining protocols with samples applied to 2D gels.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2003 (RE2) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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