Award details

Multiuser TIRF microscopy setup for realtime cellular and molecular imaging

Reference	REI20560
Principal Investigator / Supervisor	Professor Michael Ferenczi
Co-Investigators / Co-Supervisors	Dr Katherine Brown, Professor Anthony Cass, Professor Daniel Davis, Professor Paul Freemont, Dr Yoshifumi Itoh, Emeritus Professor Anthony Magee, Professor Hideaki Nagase, Professor Miguel Seabra, Professor Thierry Soldati, Dr Rudiger Woscholski
Institution	Imperial College London
Department	Div of Biomedical Sciences
Funding type	Research
Value (£)	66,796
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2004
Duration	12 months

Abstract

The application is a for a Total Internal Reflection Fluorescence (TIRF) optical microscope to provide new opportunities for cellular and molecular imaging in real time. The advantage of a TIRF microscope is its high time resolution and high sensitivity which it ideal for studying the dynamics of cells, organelles and molecules. The main projects for which the microscope will be used are as follows: 1. Investigation of the structural changes in the kinesin molecule during movement along microtubules (Prof. Michael A Ferenczi). 2. Morphology and dynamics of the endocytic and exocytic pathways (Dr.Thierry Soldati). 3. Real-time TIRF imaging of signalling at immunological synapses (Dr. Daniel Davis and Prof. Anthony Magee). 4. Study of Phosphoinositol lipids (PI) by TIRF microscopy (Dr. R. Woscholski). 5. Investigation of the role of Rab27a in organelle dynamics and secretion (Prof. Miguel Seabra and Prof. Michael Ferenczi). 6. The role of PML nuclear domains in mammalian transcriptional regulation and nuclear organisation (Prof. Paul Freemont). 7. Molecular mechanisms of metalloproteinases in the turnover of extracellular and pericellular matrix (Prof. Hideaki Nagase and Dr. Yoshi Itoh). 8. TIRF Microscopy: Applications in Micro- and Nanotechnology (Prof. Tony Cass). 9. Investigation of type III secretion in pathogenic Yersinia (Dr. Katherine Brown).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2003 (RE2) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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