Award details

Real time 3-dimensional imaging of fluorescently tagged proteins in living cells

Reference	REI18513
Principal Investigator / Supervisor	Professor Peter Edwin Sudbery
Co-Investigators / Co-Supervisors	Professor Simon J. Foster, Professor Alastair Goldman, Professor Julie Gray, Dr Lynda Partridge, Mr P Strobl, Professor Geoffrey Turner
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	102,260
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2004
Duration	12 months

Abstract

The equipment requested is for a Delta Vision Restoration microscopy System, designed to provide high-resolution, multi-wavelength fluorescent imaging. This system will be used in a variety of projects that involve time-lapse imaging in live cells, which will often be microorganisms. This means that it is essential to use low excitation and imaging to avoid photo damage to the cells and photo bleaching of the flurophore, the two key problems in this type of work. This facility will be used for the following projects: 1) The molecular mechanisms of hyphal morphogenesis in Candida albicans; 2) Molecular pathogenesis of Staphylococcus aureus infections; 3) Tracking environmental signalling pathway components in living plant cells; 4) Imaging changes within mast cells upon binding of IgE to its receptor; 5) The role of tetraspanin protein in the organisation of biological membranes; 6) The mechanism of chromosome pairing in S. cerevisiae.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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