Award details

Single nucleotide polymorphism discovery in wheat and other polyploids

Reference	REI18509
Principal Investigator / Supervisor	Professor Graham Moore
Co-Investigators / Co-Supervisors	Dr Robert Koebner
Institution	John Innes Centre
Department	Crop Genetics
Funding type	Research
Value (£)	53,932
Status	Completed
Type	Research Grant
Start date	12/06/2003
End date	12/06/2004
Duration	12 months

Abstract

The WAVE dHPLC platform is well proven in its capacity to detection mutations in human amplicons. Detection is based on the physical properties of a heteroduplex when it is passed down an HPLC column. The heteroduplexes are not fragmented (as in the Cell platform), rather they are partially denatured and thus migrate differently from a homoduplex molecule. This should allow the platform to be applicable to the major target species hexaploid wheat, since the presence of a SNP in one (or more) of the constituent amplicon species will be manifested in a change in the number (or shape) of the peaks generated from the reference amplicon. The primary application will be to characterise allelic diversity at the sequence level of the large national wheat germplasm collection held at JIC. This new programme represents a key part of JICs strategy to link model system gene sequence and genomics technology to its longstanding strength in crop genetics, in order to gain a better understanding of natural genetic variation in what is the UKs most important crop species.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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