Award details

Macromolecular assemblies: compositionally dynamic units that regulate cell behaviour

Reference	REI18506
Principal Investigator / Supervisor	Professor David Fernig
Co-Investigators / Co-Supervisors	Professor John Gallagher, Dr Malcolm Lyon
Institution	University of Liverpool
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	75,000
Status	Completed
Type	Research Grant
Start date	01/02/2003
End date	01/02/2004
Duration	12 months

Abstract

The Farfield AnaLight 200, a second generation optical biosensor, provides the means to determine the geometric relationships of molecules during the formation and the dissociation of multimolecular complexes, as well as measuring simultaneously the kinetics of a molecular interaction. This it achieves by using interferometry to measure the thickness of the adsorbing layer of molecules in real-time, with a precision of 0.1 nm. The applicants and their collaborators programme of research includes the analysis of the kinetics and geometry of the following complexes: 1) Protein-oligosaccharide and protein-polysaccharide; 2) Trimolecular and higher order, e.g. FGF/HS/FGF receptor tyrosine kinase; 3) A membrane analogue and a receptor dimerisation sensor anchored to this; 4) S100 protein complexes and non-muscle myosin microfilament growth; 5) In addition, the applicants will develop novel methodologies with Farfield Sensors, which will extend considerably the use of the instrument. The AnaLight 200 will complement the existing first generation optical biosensors and will provide the users with a novel approach to the study of the assembly of macromolecular complexes. In doing so the AnaLight will also help the establishment of future collaborations between biological and physical scientists in Liverpool. Therefore, the AnaLight will have a major impact on the quality of bioscience research in the University.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search