Award details

Accessing the bacterial 'diversome' by ultra-fast DNA sequencing

Reference	REI18469
Principal Investigator / Supervisor	Professor Peter Leadlay
Co-Investigators / Co-Supervisors	Mr John Lester
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	121,804
Status	Completed
Type	Research Grant
Start date	02/04/2003
End date	01/04/2004
Duration	12 months

Abstract

The equipment asked for is a new and greatly enhanced version of the ABI 96-capillary, automated DNA sequencer, which (in 1999) itself represented a technical breakthrough. The new instrument would replace obsolete older sequencers and would dramatically increase the throughput (without additional staff costs) of an established and highly successful DNA Sequencing Facility based in the Department of Biochemistry. The increased throughput would be used to launch specific large-scale de novo sequencing projects in support of our BBSRC funded research on the biosynthesis of antibiotic and other bioactive polyketides by actinomycetes. We have already demonstrated our capabilities in this area by accomplishing the total genomic sequencing of the erythromycin-producing Saccharopolyspora erythraea (8 Mbp). We aim to turn our attention to sequencing of large numbers of different gene clusters encoding entire pathways for polyketides and polypeptides in order to (i) assess the true nature of molecular diversity in secondary metabolites; (ii) to relate an increasing number of gene sets to the small molecules they produce; and (iii) to make available further novel genes/enzymes for use in combinatorial biosynthesis.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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