Award details

Enhanced facilities for increased throughput in macromolecular crystal structure analysis

Reference	REI18442
Principal Investigator / Supervisor	Professor Keith Wilson
Co-Investigators / Co-Supervisors	Professor Andrzej Brzozowski, Professor Gideon Davies, Professor Guy Dodson, Dr Garib Murshudov, Professor Anthony Wilkinson
Institution	University of York
Department	Chemistry
Funding type	Research
Value (£)	43,068
Status	Completed
Type	Research Grant
Start date	14/11/2002
End date	13/11/2003
Duration	12 months

Abstract

In the present proposal we wish to enhance the infrastructure for crystal structure determination in the York Structural Biology Laboratory by purchasing two items of equipment relevant to two of the bottlenecks in protein crystallography, namely crystallogenesis and X-ray data collection. Funds are sought (1) for a Cartesian crystallisation robot to allow us to screen crystal conditions more effectively and productively in a semi-automated manner and (2) for a set of Osmic mirrors to increase the throughput in our X-ray data collection suite. We have selected the Cartesian system as most cost effective and tested for our entry into the field of crystallisation robotics, having carried out a detailed survey of other laboratories already involved in such work, and attending several workshops on this topic. The device will allow us to screen large numbers of small- scale drops and make optimum use of small amounts of protein available for challenging projects, as well as being essential to our new functional genomics programmes in Malaria and C. jejuni. The Osmic mirror system is now an accepted standard for high intensity diffraction systems, and will increase the throughput on our second rotating anode/detector by roughly 3-fold, and hence almost double our data collection capacity.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search