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Automation for map based cloning transcription profiling and new applications to plant metabolite analysis

Reference	REI18439
Principal Investigator / Supervisor	Professor Iain Donnison
Co-Investigators / Co-Supervisors	Professor Michael Abberton, Professor Joseph Gallagher, Dr Catherine Howarth, Dr Mervyn Owen Humphreys, Professor Ian King, Professor Phillip Morris, Professor Howard Thomas, Dr Danny Thorogood
Institution	Inst of Grassland and Environmental Res
Department	Cell Biology Department
Funding type	Research
Value (£)	198,972
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2004
Duration	12 months

Abstract

The use of BAC and DNA-based molecular markers for gene isolation, mapping and marker assisted selection is central to IGERs multidisciplinary research which includes the dissection, mapping, marking and manipulation of complex traits in forage crops and cereals. At present BAC, lambda phage and plasmid containing bacterial colonies are picked by hand which is highly labour-intensive and restricts lab-throughput. In addition BAC, phage and plant DNA extraction for Southern or RFLP analysis is also dependent on labour intensive low-throughput methods. We are therefore applying for funds to purchase a colony picking robot and fully automated high- quality, large-scale, high-throughput DNA extraction robot. This would not only bring dramatic benefits to current research but also enable IGER to compete more effectively for genomics and functional genomics based research contracts. We also propose through customisation that the equipment be modified for metabolite extraction and analysis.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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