Award details

High sensitivity sequencing of proteins and peptides at Aberdeen proteomics facility

Reference	REI18435
Principal Investigator / Supervisor	Professor Ian Booth
Co-Investigators / Co-Supervisors	Dr Phillip Cash
Institution	University of Aberdeen
Department	School of Medical Sciences
Funding type	Research
Value (£)	71,832
Status	Completed
Type	Research Grant
Start date	17/04/2003
End date	16/04/2005
Duration	24 months

Abstract

Proteomics is a powerful tool for developing an insight into changes in protein expression in response to changes in either the genome or the environment. Sensitive mass spectrometry (MS) combined with separation of protein in two dimensions using isoelectric focussing followed by SDS-PAGE allows the identification of the individual proteins. Changes in abundance or mobility, which is often a consequence of modification, can be detected. Current methodologies rely on the application of MS techniques. However, the use of MS technologies relies on the proteins that are being characterised already having been deposited in the databases as a result of either protein or genome sequencing. Until recently, protein abundance on 2D gels was rarely sufficient for the identification by protein sequencing methods. A particular problem is that only a few proteins are sufficiently abundant for protein sequencing. Recent work in our proteomics facility has developed methods for protein enrichment, which we are developing to make available as spin column technology, that makes microsequencing a feasible approach to protein identification. In parallel, the new generation of peptide sequencers from Applied Biosystems have greatly improved sensitivity that allows sequencing of even low to moderate abundance proteins separated on 2D gels. We, therefore, propose to purchase the PROCISE CLC protein sequencing system to expand the services that we can offer to the academic and biotechnology communities. In Aberdeen this instrument will support research on bacterial and fungal pathogenesis, molecular parasitology, vector-parasite relationships, analysis of RNA- protein complexes, pathobiology of fish and human liver, analysis of gene transcription in diabetes, analysis of renal and bone disease. In addition, the Aberdeen facility supports University research groups throughout the UK and mainland Europe and a diverse range of Pharmaceutical companies, including SMEs. Thus the award of thisgrant will enable the Aberdeen research community to thrive and will continue to underpin the efforts of the wider UK community.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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