Award details

Real time live cell imaging in plants and cells

Reference	REI18430
Principal Investigator / Supervisor	Professor Peter Shaw
Co-Investigators / Co-Supervisors	Professor John Doonan
Institution	John Innes Centre
Department	Cell and Develop Biology
Funding type	Research
Value (£)	77,580
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/01/2004
Duration	9 months

Abstract

This abstract is a request for funds to purchase a newly developed real- time spinning disc/CCD confocal microscope, which will provide an essential enhancement of our capabilities for live cell imaging, a major component of many projects on this site. The equipment will be housed in the bioimaging suite in the Department of Cell and Developmental Biology, and will be available for use by all scientists on the site. It will be run and maintained by two highly skilled support staff, who will provide training to all potential users, and who will be available for trouble-shooting and advising users. The spinning disc/CCD systems overcome many of the problems with sensitivity and photodamage that have been a problem with using conventional spot-scanning confocal microscopes with living cells. Two companies currently market a suitable machine -Perkin Elmer (Ultraview) and Visitech (Vox Cell Scan). Both instruments are priced similarly, and a final decision on which to purchase will be made on a number of criteria, including value for money, ease of use and technical performance. This proposal is supported by a contribution from Syngenta, and the equipment will be used extensively in collaborative projects supported by Syngenta under the Research Collaboration agreement between JIC and Syngenta. These projects centre around the analysis of endosperm development in wheat and Arabidopsis. As other examples of projects which will use the equipment we give brief descriptions of proposed research in a number of areas of JIC science including cell cycle and cell cytoskeleton studies, studies of virus replication, imaging calcium during early symbiotic signalling, dynamic nuclear organisation and meiosis.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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