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Mapping dynamic intraplastidic localisation patterns of plastid division components in plants

Reference	REI18421
Principal Investigator / Supervisor	Professor Simon Moller
Co-Investigators / Co-Supervisors
Institution	University of Leicester
Department	Biology
Funding type	Research
Value (£)	30,864
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	31/03/2004
Duration	12 months

Abstract

We seek, as an integrated part of our BBSRC funded research programme, to use advanced fluorescence microscopy including multi channel (GFP, YFP, CFP) time-lapse in Z image capture and deconvolution analysis to map the dynamic intraplastidic localisation patterns of all isolated plastid division components in plants and to relate this to their function. We have generated numerous transgenic plants containing YFP and CFP fusion proteins and will use these to address a number of interesting issues: (1) transgenic plants containing AtMinD1/YFP, AtMinE1/YFP, AtFtsZ1-1/YFP, AtFtsZ2-1/YFP and AtSulA1/YFP will be used to analyse real-time localisation dynamics, (2) transgenic plants containing both AtMinD1/CFP and AtMinE1/YFP will be used to analyse simultaneous dynamic localisation patterns to start mapping the sequence of events during division, (3) transgenic plants deficient for one division component containing a fusion protein of another component (e.g. AtMinD1- deficient plant containing AtMinE1/YFP) will be used to assess the interdependency of the components in terms of intraplastidic localisation patterns and (4) transgenic plants with elevated levels of one division component containing a fusion protein of another component will also be used to assess interdependency. All plants described, in all the possible combinations, have been generated and once we have the requested image capture and analysis equipment we will start our studies. As we continue to isolate additional division components these will be analysed as above and integrated into our expanding dynamic intraplastidic localisation map of plastid division components. The results obtained from the proposed research will set the scene for understanding plastid functions in more detail, not only at the level of basic research but also in terms of applied agricultural applications.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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