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Differential proteomic analysis using 2D-DIGE chemiluminescence and radioactive imaging for functional genomics

Reference	REI18419
Principal Investigator / Supervisor	Professor Antony Slabas
Co-Investigators / Co-Supervisors	Professor Adam Benham, Professor Martin Cann, Professor Robert Edwards, Professor Christopher Hutchison, Professor Keith Lindsey, Professor Stefan Przyborski, Professor Roy Quinlan
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	74,000
Status	Completed
Type	Research Grant
Start date	04/12/2002
End date	03/06/2003
Duration	6 months

Abstract

This application is for the purchase of a Typhoon phosphoimager, 2D- DIGE technology and DeCyder differential dye imaging software to enable us to quantify and identify differences in protein samples using a variety of labelling systems. In particular it will allow the comparison of gels and western blots from one work station. The phosphoimager will also be used by other members of the school involved in projects, in cell biology, biochemistry and molecular biology requiring chemiluminescent and radioactive imaging. The equipment will be widely used within and outside the school to investigate the following problems: (1) Arabidopsis adaptation to biotic and abiotic stress; (2) Characterising the plant xenome; (3) Developmental biology of endoplasmic reticulum of Castor (Ricinus communis); (4) Cyanobacterial adaptation to stress and mechanism of cell signalling; (5) Plant signalling and morphogenesis; (6) Integrated down regulation of metabolism; (7) Androgen signalling in prostate cell lines; (8) Detection of nuclear proteins with altered anchorage functions in Emery Dreifuss Muscular Dystrophy Fibroblasts; (9) Stem cell differentiation and cell signalling pathways; (10) Role of chaperone-cytoskeleton inclusions in pathology; and (11) Mechanisms of oxidative protein folding.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2002 (REI) [2002]
Funding Scheme	X – not Funded via a specific Funding Scheme

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