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A study of the starvation-stress response of Salmonella enterica using MudJ fusions and DNA microarrays

Reference	PRS12194
Principal Investigator / Supervisor	Professor Mark Pallen
Co-Investigators / Co-Supervisors	Professor Brendan Wren
Institution	University of Birmingham
Department	Medical Sciences - Medicine
Funding type	Research
Value (£)	172,806
Status	Completed
Type	Research Grant
Start date	01/05/2000
End date	01/05/2003
Duration	36 months

Abstract

We propose to identify the genes involved in the starvation-stress response of Salmonella enterica, using two complementary approaches: (1) MudJ lac fusions differentially expressed during starvation and (2) cDNA hybridisation to DNA microarrays of all ORFs identified in genome sequencing. The contribution of the genes we identify to bacterial survival under environmental stress will be determined (oxidative, thermal, acid and osmotic stress; cationic peptides). Genes for which expression is dependent on loci known or suspected to regulate the starvation-stress response (rpoS, relAspoT, cya/crp, rpoH, Irp, oxyR, fadR) will be identified by comparing the transcriptional responses to stress in regulatory mutants to those in the wild type.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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