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Molecular interactions in the pathway regulating stress-specific transcription factor SigB of Bacillus subtilis

ReferencePRS12158
Principal Investigator / Supervisor Dr M Yudkin
Co-Investigators /
Co-Supervisors
Institution University of Oxford
DepartmentBiochemistry
Funding typeResearch
Value (£) 193,864
StatusCompleted
TypeResearch Grant
Start date 01/01/2000
End date 30/06/2003
Duration42 months

Abstract

SigB is regulated by a pathway consisting of several proteins arranged in two modules. The downstream module activates SigB when cells are starved. The upstream module transduced environmental signals to the downstream module. Communication between the various proteins is partly non-covalent interactions, and partly two pairs of protein kinases and phosphates. We shall study a) the dissociation constants of the non-covalent interactions; b) the intracellular concentration of each protein; c) kinetic properties of the kinases and phosphatases; d) the ratio of phosphorylated to non-phosphorylated substrates; e) phosphorylation and dephosphorylation of the protein thought to sense environmental signals and its homologues.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding SchemeX – not Funded via a specific Funding Scheme
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