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Sensing and responding to nitrogen and oxygen stress through the NIFL:NIFA two-component regulatory system

Reference	PRS12157
Principal Investigator / Supervisor	Professor Raymond Alan Dixon
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	Molecular Microbiology
Funding type	Research
Value (£)	159,824
Status	Completed
Type	Research Grant
Start date	03/04/2000
End date	02/08/2003
Duration	40 months

Abstract

The expression of bacterial operons encoding proteins required for nitrogen fixation is controlled at the level of transcription initiation in response to the level of fixed nitrogen, environmental oxygen and potentially the energy status. We intend to use a combination of physiological, biochemical and genetic approaches to understand how these stresses are perceived and integrated by the anti-activator NIFL and transduced to the transcriptional activator NIFA to control biosynthesis of a highly expressed bacterial protein under stringent conditions. This will provide a paradigm for understanding how protein complexes interact with intracellular signals to bring about changes in gene expression under conditions of nutrient limitation and may lead to the design of novel expression vectors.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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