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Is cyclic GMP a second messenger of nitric oxide stress in Paracoccus denitrificans?
Reference
PRS12156
Principal Investigator / Supervisor
Dr S Spiro
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
162,803
Status
Completed
Type
Research Grant
Start date
09/01/2000
End date
09/01/2003
Duration
36 months
Abstract
The NNR protein of P. denitrificans activates transcription in response to NO, and our data indicate that the active species of NNR is a complex with cGMP. This study will test the hypothesis that cGMP acts as a second messenger of NO exposure in P. denitrificans, and will explore the cGMP biosynthetic pathway. Cyclic GMP will be assayed in extracts of cells grown under different conditions, and the relationship between cGMP synthesis and NO production examined. Effects of exogenous cGMP and its analogues on NMR mediated gene expression will be studied. Genetic and biochemical approaches will be used to identify the genes and enzymes involved in cGMP synthesis, to determine there is no NO- activated pathway for cGMP synthesis.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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