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Sensory transduction in the activation of synthesis of pathogenicity factors by a diffusible signal molecule in Xanthomonas campestris
Reference
PRS12153
Principal Investigator / Supervisor
Dr S Spiro
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Sainsbury Laboratory
Funding type
Research
Value (£)
121,343
Status
Completed
Type
Research Grant
Start date
23/11/1999
End date
23/09/2002
Duration
34 months
Abstract
Synthesis of pathogenicity factors in Xanthomonas campestris is positively regulated by the rpf cluster of genes. Two members of this cluster, rpfF and rpfB, are involved in regulation mediated by small diffusible molecule (DSF) which is not an N-acyl homoserine lactone. The aims of this work are 1) to investigate the roles of rpfG/C/H, which encode components of a signal transduction system, in the sensing of DSF and activation of pathogenicity gene transcription. This will be done through creation of specific non-polar mutations in these genes and through the creation of mutant alleles simulating phosphorylated and non- phosphorylated forms of the regulatory proteins 2) to identify further genes required for the response to DSF through transposon mutagenesis 3) to use 2D gel electrophoresis to identify cellular proteins which are DSF-regulated.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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