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The role of MutT motif proteins in environmental adaptation in Deinococcus

Reference	PRS11831
Principal Investigator / Supervisor	Professor Alexander McLennan
Co-Investigators / Co-Supervisors	Professor Peter Strike
Institution	University of Liverpool
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	219,046
Status	Completed
Type	Research Grant
Start date	01/10/1999
End date	01/10/2002
Duration	36 months

Abstract

The radio- and desiccation resistant Deinococcus radiodurans has genes for at least 20 members of the MuT motif protein family. By analogy with the E. coli MutT protein itself, we propose the function of many of these family members to be the hydrolysis of mutagenic or toxic oxidised nucleotides generated by environmental stress. The stress- inducibility of the genes will be studied by RT-PCR and rationally selected examples of the gene products expressed in E. coli, purified and their modified nucleotide substrate specificity determined by HPLC and by mass spectrometric identification of oxidised bases after hydrolysis. The results will be related to stress adaptation in the organism and will provide information on the toxicity of the base modifications.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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