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The molecular response to environmental nitrate in Arabidopsis roots
Reference
PMB00570
Principal Investigator / Supervisor
Professor Brian Gordon Forde
Co-Investigators /
Co-Supervisors
Institution
Rothamsted Research
Department
Biological Chemistry & Crop Protection
Funding type
Research
Value (£)
17,020
Status
Completed
Type
Research Grant
Start date
01/04/1993
End date
01/04/1997
Duration
48 months
Abstract
The role of nitrate as an environmental signal molecule is well-established, but the plant's response is poorly understood at the molecular level. We propose to use a subtractive hybridization procedure to enrich for mRNAs that are rapidly (< 60 min) up- or down- regulated in Arabidopsis roots by NO3 and to clone their cDNAs. An inhibitor of protein synthesis will be used to identify primary response genes and the kinetics of mRNA induction will be studied. The specificity of the nitrate-responsive genes will be determined and used to scan sequence databases for homologous to other genes. A transgenic Arabidopsis line carrying a herbicide resistance gene under the control of a NO3 inducible promoter will be generated and used to screen for nitrate regulatory mutants.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Plant Molecular Biology II (PM2) [1986]
Funding Scheme
X – not Funded via a specific Funding Scheme
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