Award details

Cloning antibody genes for transformation of plants to obtain virus resistance and expression in bacteria to produce novel immunodiagnostic reagents

Reference	PMB00565
Principal Investigator / Supervisor	Dr DJ Nichols
Co-Investigators / Co-Supervisors	Dr W Cockburn
Institution	University of Leicester
Department	Medicine - Pathology
Funding type	Research
Value (£)	222,766
Status	Completed
Type	Research Grant
Start date	01/03/1993
End date	01/07/1997
Duration	51 months

Abstract

Antibody genes will be cloned from existing hybridomas secreting monoclonal antibodies (MAbs) specific for different coat protein epitopes on potato viruses from four virus groups. The gene sequences encoding the antigen binding sites (single-chain Fv fragments) will be expressed in bacterial systems. The specific binding affinity of the antibodies expressed in bacteria will be assessed by immunological techniques. The production of novel diagnostic reagents will be investigated. Suitable antibody genes, and combinations of antibody genes will be selected for use in Agrobacterium- mediated transformation in a model plant system, tobacco, and the transgenic plants will be tested for resistance to virus infection. If resistance can be successfully induced by this means, agronomically valuable varieties of potato will be transformed with suitable combinations of antibody genes.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Plant Molecular Biology II (PM2) [1986]
Funding Scheme	X – not Funded via a specific Funding Scheme

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