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Metabolic control of Calvin cycle genes in wheat and Arabidopsis

Reference	PMB00561
Principal Investigator / Supervisor	Professor Christine Raines
Co-Investigators / Co-Supervisors	Dr Julie Lloyd
Institution	University of Essex
Department	Biological Sciences
Funding type	Research
Value (£)	178,731
Status	Completed
Type	Research Grant
Start date	11/01/1993
End date	11/07/1997
Duration	54 months

Abstract

The aim of this work is to study the role of metabolites in the regulation of transcription of Calvin cycle genes in wheat and Arabidopsis. Northern slot blot analysis and PCR techniques will be used to determine the changes in expression of mRNA encoding FBPase, SBPase and PRKase in intact plants and protoplasts. Metabolite- responsive cis-acting elements will be identified using deletion mutants of the FBPase gene upsteam regions from wheat and Arabidopsis. This analysis will use a combination of transient expression in protoplasts and stably transformed transgenic plants. A combination of in vivo footprinting and in vitro gel-shift analysis will be used to identify and localise DNA-protein interactions.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Plant Molecular Biology II (PM2) [1986]
Funding Scheme	X – not Funded via a specific Funding Scheme

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