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Interaction of proteins with the tomato mosaic virus replicase: cloning of the tomato TM-1 resistance gene

Reference	PMB00550
Principal Investigator / Supervisor	Professor Kenneth Buck
Co-Investigators / Co-Supervisors
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	212,456
Status	Completed
Type	Research Grant
Start date	01/03/1993
End date	31/08/1997
Duration	54 months

Abstract

The tomato Tm-1 resistance gene inhibits the replication of tomato mosaic virus strain 0 (ToMV-O), but not that of strain 1 (ToMV-1) which has an altered replication protein. It is proposed to isolate the product of the Tm-1 gene (the Tm-1 protein) by its ability to interact with the TomV-0 replicase, thereby inactivating it or preventing its assembly. A cDNA clone of the protein will be obtained and sequenced. The clone will be used to transform +/+ tomato and tobacco plants to confirm that the protein confers resistance to ToMV-0, to confirm that ability to produce the protein segregates wit the Tm-1 gene in plant genetic crosses and as a probe to isolate a genomic clone of the Tm-1 gene.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Plant Molecular Biology II (PM2) [1986]
Funding Scheme	X – not Funded via a specific Funding Scheme

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