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Mapping T-DNA integrations carrying Ds elements onto the Arabidopsis genome
Reference
PAG04435
Principal Investigator / Supervisor
Professor Dame Caroline Dean
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
219,914
Status
Completed
Type
Research Grant
Start date
01/11/1995
End date
01/11/1998
Duration
36 months
Abstract
We aim to map 100 T-DNA integrations carrying Ds elements in order to improve the efficiency of targeted insertional mutagenesis in Arabidopsis. New sAc and Ds elements would be constructed that allow transposition events to be selected in the greenhouse. Landsberg erecta transformants with single copy T-DNA integrations carrying the Ds element would be generated and plant DNA flanking the insertion isolated. The flanking DNA would be hybridized to arrayed YAC clones that form the physical map covering all five chromosomes. Targeted tagging experiments would be undertaken to provide information on the number of families that need to be screened to obtain a particular mutation.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Plant & Animal Genome Analysis (PAG) [1993-1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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