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Mapping microsatellites with expressed sequences in the chicken genome: a strategy to facilitate identification of trait genes and the comparison of genome maps
Reference
PAG04434
Principal Investigator / Supervisor
Professor David Burt
Co-Investigators /
Co-Supervisors
Institution
The Roslin Institute
Department
The Roslin Institute Department
Funding type
Research
Value (£)
180,952
Status
Completed
Type
Research Grant
Start date
30/10/1995
End date
30/10/1998
Duration
36 months
Abstract
The aim of this project is to map large numbers of chicken genes to facilitate the identification of trait-gene comparison of genome maps. To achieve this we will use an efficient magnetic-bead-DNA-capture procedure to isolate cDNA copies of pre-sliced RNA transcripts containing microsatellite sequences. RNAs will be isolated from tissues with a wide representation of mRNA sequences. From the sequence of cDNAs we will (i) design primer-pairs for PCR assays to detect and map microsatellites onto the chicken genome, and (ii) seek evidence for possible function using the cDNA sequences to probe a range of databases. Linkage results will be summarised in the chicken genome database and used to seek homologies with other species (Comparative Genome Mapping).
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Plant & Animal Genome Analysis (PAG) [1993-1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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